Molecular and Morphological Characterization of a Taxol-Producing Endophytic Fungus, Gliocladium sp., from Taxus baccata
نویسندگان
چکیده
The endophytic fungal populations of different tissues of Taxus baccata grown at high altitudes in West Bengal, India were explored. These isolated fungal populations represented different genera, which were screened for taxol production using immunoassay technique. The culture AAT-TS-4(1) that produced taxol was identified as Gliocladium sp. based on its cultural, morphological characteristics, internal transcribed spacer, and 18S rRNA sequence analysis. Kinetics of taxol production as a function of culture growth were investigated.
منابع مشابه
Identification of a taxol-producing endophytic fungus EFY-36
Morphological and molecular methods were used to identify the statues of an isolate, EFY-36, a taxolproducing endophytic fungus. Based on the morphology of the fungal colony, the mechanism of spore production and the characteristics of the spores, the isolate is Mucor spp. Furthermore, the analysis of 18S ribosome RNA sequence of the isolate was achieved using PCR technology. The results showed...
متن کاملHazel (Corylus avellana L.) as a New Source of Taxol and Taxanes
Taxol is used to treatment of variety cancers including ovarian, breast, lung, head and neck carcinomas and the AIDS-related Karposi’s carcinoma, and was originally obtained from bark of taxus brevifolia. However the amount of extracted taxol from taxus is extremely low and cannot be considered as a suitable commercial source and it has led to studies for finding new sources of Taxol. In 1998, ...
متن کاملFor yew and me
Endophytic fungi live within the tissues of their host plants but, even for fungi, there's no such thing as a free lunch. Some of them pay the rent by producing compounds that protect the host against invading pathogenic microbes. These scanning electron micrographs show three species of endophytic fungus that have mutually beneficial relationships with plants of the family Taxaceae (yews and t...
متن کاملMolecular Cloning and Characterization of the Phenylalanine Aminomutase Gene From Taxus baccata L.
Background: Taxol is one of the most important anti-cancer drugs, which is obtained from yew trees (<span style="font-variant: normal; ...
متن کاملInteractions between Co-Habitating fungi Elicit Synthesis of Taxol from an Endophytic Fungus in Host Taxus Plants
Within a plant, there can exist an ecosystem of pathogens and endophytes, the latter described as bacterial and fungal inhabitants that thrive without causing disease to the host. Interactions between microbial inhabitants represent a novel area of study for natural products research. Here we analyzed the interactions between the fungal endophytes of Taxus (yew) trees. Fungal endophytes of Taxu...
متن کامل