The use of isotopic carbon in a study of the metabolism of anthranilic acid in Neurospora.
نویسندگان
چکیده
The finding by Tatum, Banner, and Beadle (l), that the tryptophanless Neurospora mutant strain 10575 accumulates anthranilic acid, which in turn can be utilized for growth of strain 40008, has provided evidence that anthranilic acid is a biochemical precursor of tryptophan in this organism. It has been further established that indole is an intermediate in this conversion (2-5). More recent work with a number of mutants of Neurospora (6-8) has established that tryptophan is a biochemical precursor to niacin with kynurenine and hydroxyanthranilic acid as intermediates. The accumulated evidence has indicated the existence in the mold of the following series of reactions:
منابع مشابه
Incorporation of carbon atoms 1 and 6 of glucose into protocatechuic acid by Neurospora.
The mechanism of biosynthesis of the simpler aromatic compounds has been significantly clarified recently by the use of mutant strains of Neurospora (1) and Escherichia coli (2) and by the use of isotopic carbon (3-6). The evidence indicates that aromatization takes place via dehydroquinic acid, dehydroshikimic acid, and shikimic acid. Although the ultimate source of the carbon atoms of the aro...
متن کاملKynureninase from Neurospora: Purification
As part of a study of kynurenine metabolism in Neurospora crassa, the enzyme, kynureninase, has been investigated. Evidence from studies with mycelial pads suggests that tryptophan gives rise to kynurenine, which may be degraded to anthranilic acid (1). Kynurenine is also oxidized to hydroxykynurenine, which is converted to hydroxyanthranilic acid (2). The similarity of these reactions, i.e. th...
متن کاملAn isotopic study of the conversion of anthranilic acid to indole.
Anthranilic acid and indole have been implicated as intermediates in the biosynthesis of tryptophan in several microorganisms (l-5). A mechanism of tryptophan synthesis from indole has been established, namely the pyridoxal phosphate-dependent coupling of indole and L-serine (6-9). Previous studies of the conversion of anthranilic acid to the indole ring of tryptophan have shown that the amino ...
متن کاملThe Conversion of Shikimic Acid to Anthranilic Acid by Extracts of Neurospora Crassa.
The role of shikimic acid as a common intermediate in the synthesis of phenylalanine, tyrosine, tryptophan, and p-aminobenzoic acid has been recognized for some time (l-3). The enzymes which catalyze the synthesis of shikimic acid from intermediates of carbohydrate metabolism have been observed in extracts of bacteria (4-6) as well as Neurospora crassa (7). On the other hand, the reactions lead...
متن کاملPlant sink-source relationships and carbon isotopic labeling techniques . Taher Barzegar* and Fatemhe Nekounam
The concept of source and sink strength is presently well-recognized and accepted by the scientific community as a pertinent approach describing the mechanisms of carbohydrate partitioning into the different and competing organs at a whole plant or canopy scales. Sink–source relationships have a clear role in the size of sink organs. Besides the effect on organ size, sink/source ratio might also...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 179 2 شماره
صفحات -
تاریخ انتشار 1949