Transcriptional regulation of GATA-3 by an intronic regulatory region and fetal liver zinc finger protein 1.
نویسندگان
چکیده
GATA-3 is a T cell-specific transcription factor and is essential for the development of the T cell lineage. The transcriptional regulation of GATA-3, however, remains elusive. In this study, we report the identification of a regulatory region located within the first intron of the murine GATA-3 gene. The intronic regulatory region contains both a positive and a negative cis-acting element but, as a whole, serves as a potent T cell-specific enhancer and is essential for the promoter activity in vitro. By using yeast one-hybrid screening, we discovered that fetal liver zinc finger protein 1 (Fliz1) could bind specifically to the negative cis-acting element, the sequence of which is conserved between the mouse and human GATA-3 genes. More importantly, overexpression of Fliz1 repressed the expression of GATA-3 in vivo and in vitro. Our data suggest that the expression of GATA-3 might be partly regulated by the intronic regulatory region and Fliz1 in a developmental stage-specific fashion.
منابع مشابه
Zinc Finger Protein 1 Intronic Regulatory Region and Fetal Liver Transcriptional Regulation of GATA - 3 by an Eun
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of April 14 , 2017 . This information is current as Zinc Finger Protein 1 Intronic Regulatory Region and Fetal Liver Transcriptional Regulation of GATA - 3 by an Eun
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Zinc Finger Protein 1 Intronic Regulatory Region and Fetal Liver Transcriptional Regulation of GATA
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Finger Protein 1 Intronic Regulatory Region and Fetal Liver Transcriptional Regulation of GATA
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Self-association of the erythroid transcription factor GATA-1 mediated by its zinc finger domains.
GATA-1, the founding member of a distinctive family of transcription factors, is expressed predominantly in erythroid cells and participates in the expression of numerous erythroid cell-expressed genes. GATA-binding sites are found in the promoters and enhancers of globin and nonglobin erythroid genes as well as in the alpha- and beta-globin locus control regions. To elucidate how GATA-1 may fu...
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ورودعنوان ژورنال:
- Journal of immunology
دوره 169 1 شماره
صفحات -
تاریخ انتشار 2002