Structural dynamics of bio-macromolecules by NMR: the slowly relaxing local structure approach.
نویسندگان
چکیده
0079-6565/$ see front matter 2010 Elsevier B.V. A doi:10.1016/j.pnmrs.2010.03.002 Abbreviations: AK, adenylate kinase; AKeco, adeny anisotropy; 3D GAF, 3-dimensional Gaussian axial fluc resonance; FPK, Fokker–Planck–Kramers; GB3, the B headpiece subdomain protein; iRED, isotropic reorien free; MOMD, microscopic order macroscopic disorde Overhauser enhancement; PCA, principal component a L; RDC, residual dipolar coupling; RNA, ribonucleic ac * Corresponding author. Tel.: +972 3 5318049; fax ** Corresponding author. *** Corresponding author. E-mail addresses: [email protected] (E. Meirov 2010 Elsevier B.V. All rights reserved.
منابع مشابه
Structural Biology by NMR: Structure, Dynamics, and Interactions
The function of bio-macromolecules is determined by both their 3D structure and conformational dynamics. These molecules are inherently flexible systems displaying a broad range of dynamics on time-scales from picoseconds to seconds. Nuclear Magnetic Resonance (NMR) spectroscopy has emerged as the method of choice for studying both protein structure and dynamics in solution. Typically, NMR expe...
متن کاملProtein dynamics from NMR: the slowly relaxing local structure analysis compared with model-free analysis.
(15)N-(1)H spin relaxation is a powerful method for deriving information on protein dynamics. The traditional method of data analysis is model-free (MF), where the global and local N-H motions are independent and the local geometry is simplified. The common MF analysis consists of fitting single-field data. The results are typically field-dependent, and multifield data cannot be fit with standa...
متن کاملMethyl dynamics in proteins from NMR slowly relaxing local structure spin relaxation analysis: A new perspective.
NMR spin relaxation of (2)H nuclei in (13)CH(2)D groups is a powerful method for studying side-chain motion in proteins. The analysis is typically carried out with the original model-free (MF) approach adapted to methyl dynamics. The latter is described in terms of axial local motions around, and of, the methyl averaging axis, mutually decoupled and independent of the global motion of the prote...
متن کاملCombining NMR and small angle X-ray scattering for the study of biomolecular structure and dynamics
Small-angle X-ray scattering (SAXS) and Nuclear Magnetic Resonance (NMR) are established methods to analyze the structure and structural transitions of biological macromolecules in solution. Both methods are directly applicable to near-native macromolecular solutions and allow one to study structural responses to physical and chemical changes or ligand additions. Whereas SAXS is applied to eluc...
متن کاملIntelligent identification of vehicle’s dynamics based on local model network
This paper proposes an intelligent approach for dynamic identification of the vehicles. The proposed approach is based on the data-driven identification and uses a high-performance local model network (LMN) for estimation of the vehicle’s longitudinal velocity, lateral acceleration and yaw rate. The proposed LMN requires no pre-defined standard vehicle model and uses measurement data to identif...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Progress in nuclear magnetic resonance spectroscopy
دوره 56 4 شماره
صفحات -
تاریخ انتشار 2010