Enzyme-linked immunosorbent assay compared with neutralization tests for evaluation of live mumps vaccines.
نویسندگان
چکیده
Mumps-specific antibody levels before and after vaccination with live mumps vaccines were determined by enzyme-linked immunosorbent assay (ELISA) and neutralization tests. A correlation was found between neutralization titers and optical density in ELISA. However, postvaccination sera from some vaccinees who failed to seroconvert by neutralization contained significant levels of mumps-specific antibody detectable by ELISA. In some of these serum specimens, the antibody directed to the F polypeptide of mumps virus was predominant. Most sera positive in ELISA neutralized mumps virus upon the addition of fresh guinea pig serum to the virus-serum mixture.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 19 1 شماره
صفحات -
تاریخ انتشار 1984