RNA-guided genome editing of mammalian cells.
نویسندگان
چکیده
The microbial CRISPR-Cas adaptive immune system can be harnessed to facilitate genome editing in eukaryotic cells (Cong L et al., Science 339, 819-823, 2013; Mali P et al., Science 339, 823-826, 2013). Here we describe a protocol for the use of the RNA-guided Cas9 nuclease from the Streptococcus pyogenes type II CRISPR system to achieve specific, scalable, and cost-efficient genome editing in mammalian cells.
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عنوان ژورنال:
- Methods in molecular biology
دوره 1114 شماره
صفحات -
تاریخ انتشار 2014