Tanshinone II--a inhibits angiogenesis through down regulation of COX-2 in human colorectal cancer.

نویسندگان

  • Li-Hong Zhou
  • Qiang Hu
  • Hua Sui
  • Shu-Jun Ci
  • Yan Wang
  • Xuan Liu
  • Ning-Ning Liu
  • Pei-Hao Yin
  • Jian-Min Qin
  • Qi Li
چکیده

Angiogenesis plays a significant role in colorectal cancer (CRC) and cyclooxygenase-2 (COX-2) appears to be involved with multiple aspects of CRC angiogenesis. Our aim was to investigate the inhibitory effects of Tan II-A (Tanshinone II-A, Tan II-A) on tumor growth in mice, as well as alteration of expression of COX-2 and VEGF in CRC. We established the mice xenograft model of C26 CRC cell line, and injected 0.5, 1, 2mg/kg of Tan II-A and 1mg/kg of 5-FU in respectively in vivo. Then, we assayed tumor weight and volume, and evaluated microvascular density and expression of VEGF. COX-2 promoter and COX-2 plasmids were transfected into HCT-116 cells, followed by detection of COX-2 promoter activity by chemiluminescence, and detection of COX-2 mRNA expression by fluorescence quantitative PCR. Taken together, the results showed Tan II-A could inhibit tumor growth and suppress the VEGF level in vivo. HCT-116 cell experiments showed marked inhibitory effects of Tan II-A on COX-2 and VEGF in a dose-dependent manner. The results indicate that Tan II-A can effectively inhibit tumor growth and angiogenesis of human colorectal cancer via inhibiting the expression level of COX-2 and VEGF.

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عنوان ژورنال:
  • Asian Pacific journal of cancer prevention : APJCP

دوره 13 9  شماره 

صفحات  -

تاریخ انتشار 2012