Isolation of Cellulolytic Bacteria
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Anaerobic cellulolytic bacteria from wetwood of living trees.
Obligately anaerobic, mesophilic, cellulolytic bacteria were isolated from the wetwood of elm and maple trees. The isolation of these bacteria involved inoculation of selective enrichment cultures with increment cores taken from trees showing evidence of wetwood. Cellulolytic bacteria were present in the cores from seven of nine trees sampled, as indicated by the disappearance of cellulose from...
متن کاملEnumeration and isolation of cellulolytic and hemicellulolytic bacteria from human feces.
The fibrolytic microbiota of the human large intestine was examined to determine the numbers and types of cellulolytic and hemicellulolytic bacteria present. Fecal samples from each of five individuals contained bacteria capable of degrading the hydrated cellulose in spinach and in wheat straw pretreated with alkaline hydrogen peroxide (AHP-WS), whereas degradation of the relatively crystalline...
متن کاملIsolation and identification of a cellulolytic Enterobacter from rumen of Aceh cattle
Aims The aim of this study was to isolate and identify a cellulolytic bacterium from the rumen fluid of Aceh's cattle. Biodegradation by cellulolytic rumen bacteria can be used as a source of cellulolytic bacteria that act to degrade feed fibrous material so as to improve the quality of nutrients and digestibility of feed ingredients at a cheaper price than the use of commercial cellulase enzym...
متن کاملIsolation, Identification and Metabolic Characteriz- Ation of Cellulolytic Enzyme Producing Bacteria from Banana Pseudostem Waste Using Phenotypic Microarray Technology
The purpose of this study was to isolate cellulolytic enzyme producing bacteria which may able to utilize banana pseudostem as sole carbon source for production of cellulolytic enzymes. This was attempted by isolating 10 bacteria from banana pseudostem waste. Out of the screened, isolate NAULH-5 showed high cellulolytic activity. The isolate NAULH-5 produced highest 5.97 U/mg endoglucanase, 2.2...
متن کاملIsolation and Identification of Cellulolytic Bacteria from the Gut of Holotrichia parallela Larvae (Coleoptera: Scarabaeidae)
In this study, 207 strains of aerobic and facultatively anaerobic cellulolytic bacteria were isolated from the gut of Holotrichia parallela larvae. These bacterial isolates were assigned to 21 genotypes by amplified ribosomal DNA restriction analysis (ARDRA). A partial 16S rDNA sequence analysis and standard biochemical and physiological tests were used for the assignment of the 21 representati...
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