Improved determination of transketolase activity in erythrocytes.

THE ISOLATION OF ENZYME TRANSKETOLASE FROM HUMAN ERYTHROCYTES: THE CHARACTERIZATION OF ITS QUARTERNARY STRUCTURE

Human erythrocyte transketolase (sedoheptulose-7-phosphate: D-glyceraldehyde-3-phosphate, glycolaldehyde transferase, E.C. 2.2.1.1.) has been isolated from erythrocytes with a specific activity of 59.84 U/mg. SDS-PAGE and SE-HPLC were used both as a measure of purity and as a preparative mean to obtain a higher degree of purity. Four protein bands corresponding to molecular weights of 32,0...

Erythrocyte transketolase activity measured on the Gemstar discrete analyser

Erythrocyte transketolase activity in patients with diabetic and alcoholic neuropathies.

INTRODUCTION The activity of erythrocyte transketolase induced by thiamine pyrophosphate and normalized to age of the patient is a marker of thiamine metabolism disturbances with pathological consequences in the central and peripheral nervous system. The measurement of erythrocyte transketolase activity enables evaluation of the thiamine status and therapeutic decisions in the disorders of the ...

A continuous-flow (AutoAnalyzer II) procedure for measuring erythrocyte transketolase activity.

In this semiautomated method, an AutoAnalyzer II is used to measure the enzymic production of glyceraldehyde 3-phosphate in hemolysates, to assay erythrocyte transketolase (EC 2.2.1.1) activity. Hemolysate and indicator reactions are separated by dialysis to eliminate hemoglobin interference and increase sensitivity. Internal standards of glyceraldehyde 3-phosphate in hemolysate carriers were q...

Studies of transketolase abnormality in Alzheimer's disease.

The partially purified transketolase from each of eight well-nourished patients with Alzheimer's disease contained significantly less heat-stable component with a significantly longer half-life of heat inactivation than that from eight controls. Immunochemical studies utilizing antibodies to the purified human liver transketolase did not distinguish between red blood cell transketolases of pati...