Response to Shusterman and to Mapp

نویسنده

  • William Meggs
چکیده

-To test the identity of human tumour clonogenic cells and stem cells, a procedure was developed to allow quantitation of self-renewal capacity of human ovarian carcinoma clonogenic cells. Primary colonies grown from malignant effusions of 10 patients were disaggregated and replated; secondary colonies were observed to be similar to primary colonies in size, morphology and culture requirements. Density-gradient separation of tumour-cell populations demonstrated that not all primary clonogenic cells are capable of self-renewal during clonal expansion. Patient-to-patient variation in self-renewal capacity was shown to be significantly correlated with the concentration of the tumour-cell population in the effusion fluid, and preliminary evidence of a progressive increase in self-renewal was found in one patient. It was concluded that some, but not all, ovarian-tumour clonogenic cells have the stem-cell property of self-renewal, and that quantitation of such a property may identify an important prognostic variable.

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عنوان ژورنال:
  • Environmental Health Perspectives

دوره 102  شماره 

صفحات  -

تاریخ انتشار 1994