How KPL Purifies Its Antibodies
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چکیده
Antibodies are a key element in immunoassays They are the driving force behind specificity and sensitivity. Affinity purification of antiserum yields antibodies with the ultimate in specificity and sensitivity. It eliminates all other potentially interfering serum proteins and antibodies that will increase the background and cause cross-reactivity. It leaves behind a population of antibodies that only react with the epitopes chosen for inclusion on the affinity matrix. The flexibility of engineering the affinity matrix to include or eliminate certain epitopes means that the antibodies KPL provides have only the reactivities that we specify, while cross reactions are reduced to a defined and reproducible minimum.
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