Microfluidic Strategy for Spatiotemporally Resolved Molecular Sampling from Live Ovary Slices
نویسندگان
چکیده
A prototype microfluidic platform has been developed for spatially and temporally resolved sampling of biomarkers expressed by organotypic tissue slices. The device draws liquid from the sample reservoir through discrete sampling ports into separate analysis microchannels. By monitoring the concentrations of specific biomarkers it is possible to map the concentration profiles of these molecules within the tissue as a function of time. This study uses ovary slices to enable monitoring of key regulators of follicular maturation and ovulation including growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15), activin, FGF8, inhibin, follistatin, and betacellulin.
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