Polymorphisms in the pfcrt and pfmdr1 genes in Plasmodium falciparum isolates from South Sumatera, Indonesia
نویسندگان
چکیده
Metode: Studi molekuler dilakukan untuk mengidentifikasi alel mutan dua gen yang berhubungan dengan resistensi klorokuin pada isolat P. falciparum di Sumatera Selatan. Sebanyak 25 pasien diambil darahnya, kemudian dilakukan isolasi DNA. Susunan dari kedua gen (Plasmodium falciparum chloroquine resistance transporter/pfcrt dan Plasmodium falciparum multidrug resistance/pfmdr1) dianalisis dengan menggunakan polymerase chain reaction (PCR) dan restriction fragment length polymorphism (RFLP).
منابع مشابه
Molecular epidemiology of Plasmodium falciparum resistance to antimalarial drugs in Indonesia.
The extent of gene polymorphisms associated with resistance to chloroquine and sulfadoxine-pyrimethamine was examined in field isolates of Plasmodium falciparum from Indonesia. Eight malaria-endemic areas, representing a broad region of the western and eastern Indonesian Archipelago were surveyed. Blood from 20-50 patients was collected at each site, DNA was isolated, and the sequences of four ...
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