Visualization of the intracellular location and stability of DNA origami with a label-free fluorescent probe.

نویسندگان

  • Xibo Shen
  • Qiao Jiang
  • Jinye Wang
  • Luru Dai
  • Guozhang Zou
  • Zhen-Gang Wang
  • Wei-Qiang Chen
  • Wei Jiang
  • Baoquan Ding
چکیده

We report a label-free fluorescent strategy to study the distribution and stability of DNA origami nanostructures in live, cellular systems, using carbazole-based biscyanine as a probe molecule which has the characteristic property of restriction of intramolecular rotation (RIR) induced emission.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation and Characterization of Free and Immobilized Acethylcholinesterase with Fluorescent Probe, Differential Scanning Calorimetry and Docking

Acetylcholinesterase (AChE) enzyme which catalyses the hydrolysis of choline esters, such as acetylcholine, is very important in nerve function. Previous structural studies showed the possible amyloid fibril formation on the AChE. Therefore it is important to understand interaction of ligands to prevent the formation of  amyloid fibrils. The purpose of the present study was to  char...

متن کامل

Designing a Label Free Aptasensor for Detection of Methamphetamine

A label-free electrochemical nucleic acid aptasensor for the detection of methamphetamine (MA) by the immobilization of thiolated self-assembled DNA sequences on a gold nanoparticles-chitosan modified electrode is constructed. When MA was complexed specifically to the aptamer, the configuration of the nucleic acid aptamer switched to a locked structure and the interface of the biosensor changed...

متن کامل

Flow Visualization by Conditional Sampling of a Single X-Wire Probe in a Very Long Run Experiment

Flow visualization techniques using tracer markers such as die, smoke, hydrogen bubbles, etc., have been widely used in experimental investigations of large scale structures of a variety of flow fields. They have played an important role in understanding the physics of the coherent structures' formation and evolution in the transitional as well as the turbulent regions of the flow fields. Howev...

متن کامل

DNA origami: a history and current perspective.

Researchers have been using DNA for the rational design and construction of nanoscale objects for nearly 30 years. Recently, 'scaffolded DNA origami' has emerged as one of the most promising assembly techniques in DNA nanotechnology with a broad range of applications. In the past two years alone, DNA origami has been used to assemble water-soluble probe tiles for label-free RNA hybridization, t...

متن کامل

Flow Visualization by Conditional Sampling of a Single X-Wire Probe in a Very Long Run Experiment

Flow visualization techniques using tracer markers such as die, smoke, hydrogen bubbles, etc., have been widely used in experimental investigations of large scale structures of a variety of flow fields. They have played an important role in understanding the physics of the coherent structures formation and evolution in the transitional as well as the turbulent regions of the flow fields. Howeve...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chemical communications

دوره 48 92  شماره 

صفحات  -

تاریخ انتشار 2012