Rapid selection of high-affinity binders using ribosome display.
نویسندگان
چکیده
Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affinity binders from libraries of folded proteins. It has been successfully applied to single-chain Fv fragments of antibodies and alternative scaffolds, such as Designed Ankyrin Repeat Proteins (DARPins). High-affinity binders with new target specificity can be obtained from highly diverse DARPin libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affinity maturation and off-rate selection are explained in detail.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 805 شماره
صفحات -
تاریخ انتشار 2012