III Vitro Germplasm Conservation of Atropa baetica by Cold Storage
نویسنده
چکیده
Gerlllplaslll 01" ;/1 v;tro derived plantlels of Atroptl baeticfl was lIlainlained by cold storage al 4°C in darkness for six 1Il0nths. Tite response of Ihe cold stored gcrlllplasm to growing culture co nditions was sludied on Murasltige & Skoog mediullI supplelllented wilh BAP (0.66 mg r ), NAA (0.24 mg r\ inosilol (100 mg r ), Ihiamine (1 mg 1'1) :Hul 3% sucrose durillg a six month periodo Tlle sur"iva! rate of Ihe germplasm was similar lo lile control (pl:lllt m:rterial growll und er normal culture conditions), with all average pcrecnt ¡¡ge of 97.9 over the six month periodo The number of buds protlueed was similar for both control alld eold stOl"ed germplasm wHh an average 4.53 buds per explanl during Ihe six month period o Similarly, the number of shoots per ex plant did not differ betweell eOlilrol :lIId treatmenl. However, shoot length of eold stored gerlllplaslll \Vas slatistica lly shorler for Ihe 1" all(l 5'monlh although Ihe ovcrall six month measurcmellts showcd 110 dirrcrcllec.
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