Controlled Light Exposure Microscopy (CLEM): Photobleaching and Phototoxicity Reduced
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چکیده
Controlled Light Exposure Microscopy (CLEM): Photobleaching and Phototoxicity Reduced Photobleaching and phototoxicity are often the Achilles‘ heels of fluorescence live-cell imaging. Controlled Light Exposure Microscopy (CLEM) is a novel, simple imaging approach that reduces photobleaching and phototoxicity 2 to 10-fold without compromising image quality. The basic concept of CLEM is that light is only used where it is needed by spatial control (pixel by pixel) of the light exposure time. Experiments show that CLEM results in a 2 to 10-fold reduction of photobleaching and phototoxicity. As a bonus, CLEM increases the dynamic range of the fluorescence intensity at least by a factor of two.
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