Hpv Genotyping in Recurrent Respiratory Papillomatosis

Recurrent respiratory papillomatosis is a disease cause by Human papillomavirus (HPV) characterized by the development of papillomas in the aero-digestiv tract. About 95% of the papilloma have laryngeal localization involving HPV genotypes 6 and 11 and the rest are distributed in the nasopharynx, oropharynx, lungs, bronchi and trachea. Our research consists of a prospective clinical study on 65 patients. Inclusion criteria were: children and adult patients, positive diagnosis of RRP, patients with a history and treatment records, patients who have not performed viral analysis for RRP. Exclusion criteria were: immunodeficient patients, pregnancy and lactation, associated diseases with contraindication treatment. The principle of genotyping kit is based on the amplification of a consensus sequence of the viral genome -L1 gene. Viral genotyping was performed in all patients: 20 had genotype 6; 11 patients had genotype 11; 2 patients had genotype 16; 2 patients had genotype 18; 2 patients had genotype 30; 2 patients had HPV 32; 3 patients had genotype 40; 1 case is with HPV 55; 1 person with HPV 2 and 21 patients were HPV negativ. Identification of genotype help us to classify “high-risk” disease on progression to malignancy and to choose the optimal therapeutic scheme surgery and antiviral therapy.