The proteolytic system involving calpains.

Autophagosomes were isolated from perfused livers on Percoll gradients after the administration of cathepsin inhibitors (Kominami e t aZ., 1983). Cycloheximide (1.8 X lo-' M ) or vinblastine ( 1 X M ) were administered either simultaneously or 60 min after the protease inhibitors. ( a ) Acid phosphatase accumulation in autophagosomes (units/g of liver); ( b ) protein accumulation in autophagosomes (mg/g of liver). 0----0, Control; A--.-n, cycloheximide at zero time; A---A, cycloheximide at 60 min; m. , vinblastine at 60 min.