High human cytomegalovirus pp65 level in cardiac allografts precedes acute rejection episodes
نویسندگان
چکیده
Cytomegalovirus (CMV) infection is a risk factor for acute and chronic rejection of transplanted organs. We evaluated, using immunohistochemistry, the presence of CMV pp65 matrix protein in cardiac allografts and its association with acute rejection. CMV pp65 was found in 83/88 (94%) endomyocardial biopsies obtained from 35 patients and the presence of CMV was confirmed in selected patients by a nested polymerase chain reaction (PCR) assay. Although no association was found between CMV pp65 levels and rejection grade, high CMV pp65 levels preceded acute rejection episodes (p=0.001) indicating a possible role for CMV intra graft infection in the pathogenesis of allograft rejection.
منابع مشابه
Design of cocktail peptide vaccine against Cytomegalovirus infection
Objective(s):Human Cytomegalovirus (HCMV) remains a major morbidity and mortality cause in immuno suppressed patients. Therefore, significant effort has been made towards the development of a vaccine. In this study, the expression of the pp65 and gB fusion peptides and Fc domain of mouse IgG2a as a novel delivery system for selective uptake of antigens by antigen-presenting cells (APCs) in Pich...
متن کاملIntragraft interleukin 2 mRNA expression during acute cellular rejection and left ventricular total wall thickness after heart transplantation.
OBJECTIVE To assess whether diastolic graft function is influenced by intragraft interleukin 2 (IL-2) messenger RNA (mRNA) expression in rejecting cardiac allografts. DESIGN 16 recipients of cardiac allografts were monitored during the first three months after transplantation. The presence of IL-2 mRNA in endomyocardial biopsies (n = 123) was measured by reverse transcriptase polymerase chain...
متن کاملAccelerated rejection of murine cardiac allografts by murine cytomegalovirus-infected recipients. Lack of haplotype specificity.
Clinical studies have revealed a correlation between cytomegalovirus (CMV) infection and acute allograft rejection. Likewise, for a murine model we observed that C3H (H-2k) recipients infected with murine CMV (MCMV) rejected BALB/c (H-2d) cardiac allografts earlier than uninfected recipients (6.9 +/- 0.1 d compared with 8.1 +/- 0.6 d; P < 0.001). It has been hypothesized that MCMV epitopes cros...
متن کاملT cell recognition patterns of immunodominant cytomegalovirus antigens in primary and persistent infection.
Replication of human cytomegalovirus is controlled by a vigorous CD8 T cell response. The persistent nature of infection is believed to periodically stimulate T cell responses resulting in considerable expansions of virus-specific CD8 T cells over time. In this study, we describe the magnitude and breadth of CD8 T cell responses against the immunodominant viral Ags, IE-1 and pp65, in acute and ...
متن کاملTargeted preemptive therapy according to perceived risk of CMV infection after kidney transplantation.
BACKGROUND The identification of the best strategy to manage cytomegalovirus infection is hampered by uncertainties regarding the risk/benefit ratios of universal prophylaxis versus preemptive therapy, the impact of indirect cytomegalovirus effects and the associated costs. This study investigated the efficacy and safety of targeted preemptive therapy according to perceived risk of cytomegalovi...
متن کامل