Maintenance of a certain rumen protozoal population in a continuous in vitro fermentation system.

Thymus kotschyanus Essential Oil Components and Their Effects on in vitro Rumen Fermentation, Protozoal Population and Acidosis Parameters

This study aimed to identify chemical composition of Thymus kotschyanus essential oil (TKEO) and to evaluate the effects of different doses of TKEO on in vitro gas production, fermentation parameters, acidosis and protozoal population using a completely randomized design with four replicates. Two diets (D1: 100% forage and D2: 30% forage+70% concentrate) were incubated with buffered rumen fluid...

Effects of a diet containing whole cottonseed on rumen protozoal population and fermentation parameters in sheep

Modifications of a Device for Maintenance of the Rumen Microbial Population in Continuous Culture.

An improved and simplified apparatus for maintaining the rumen microbial population in continuous culture was constructed. All components were easily obtained from commercial sources or were simple to construct. Mechanical difficulties were minimal, and little attention was needed on the part of the operator. The deoxyribonucleic acid (DNA) content of the cultures (100 to 150 mug/ml) varied lit...

In vitro Effects of Exogenous Fibrolytic Enzymes on Rumen Fermentation of Wild Guinea Grass (Panicum maximum)

This study aimed to evaluate the potential of commercial enzymes cellulase (CE), xylanase (XY) and the combination of CE + XY to improve rumen fermentation of Guinea grass ecotype ‘A’ (Panicum maximum). The experiment was carried out in a randomized complete block design. In vitro incubations were performed with four doses of individual enzymes and their combinations (1:1 from each enzyme) as 5...

Effects of Thyme Essential Oil and Disodium Fumarate on Ruminal Fermentation Characteristics, Microbial Population and Nutrient Flow in a Dual Flow Continuous Culture System

The aim of the present study was to investigate the effects of di-sodium fumarate (DSF) and thyme essential oil (TEO) solely and simultaneously on ruminal fermentation properties and microbial abundance. A dual-flow continuous culture system (DFCC) with eight 1400-mL fermenters was used in a period of 12 d that divided to 9 d for adaptation and 3 d for sampling. Fermenters were fed 100 g d...