Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci.

نویسندگان

  • R Kariyama
  • R Mitsuhata
  • J W Chow
  • D B Clewell
  • H Kumon
چکیده

Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 38 8  شماره 

صفحات  -

تاریخ انتشار 2000