Electrophoresis of Lactate Dehydrogenase Isoenzymes ITXd 1
نویسندگان
چکیده
SubmitDoris McKenzie and A. Ralph Henderson, Deters: partment of Clinical Biochemistry, University Hospital (University of Western Ontario), London, Ontario, Canada N6A 5A5 EvaluaStanley E. Gordesky, Division of Laboratories, tors: Albert Einstein Medical Center, Philadelphia, PA 19141 Lilian M. Ewen, Department of Clinical Biochemistry, Royal Columbian Hospital, New Westminster, British Columbia, Canada V3L 3W7 Derk A. Vloedman, Department of Laboratories, Mercy Hospital, Wilkes-Barre, PA 18765 Assigned Raymond E. Vanderlinde, Department of PaEditor: thology and Laboratory Medicine, Hahnemann Medical College & Hospital of Philadelphia, Philadelphia, PA 19102
منابع مشابه
Simultaneous separation of serum creatine kinase and lactate dehydrogenase isoenzymes by ion-exchange column chromatography.
Lactate dehydrogenase isoenzymes were partially separated by use of a previously described column technique for creatine kinase [Clin. Chem. 20, 36 (1974)]. Extracts of lactate dehydrogenase-rich tissues were used to evaluate column resolution. Samples layered on mini-columns containing DEAE-Sephadex were eluted with Tris-buffered sodium chloride (100 and 200 mmol/liter). Lactate dehydrogenase ...
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Five lactate dehydrogenase (LD; L-lactate:NAD oxidoreductase, EC 1.1.127) isoenzymes are demonstrated by electrophoresis in normal human serum. Altered activities of these isoenzymes have been associated with pathological conditions, so that their determination has become a useful diagnostic aid. Abnormal LD-isoenzyme patterns with one and two extra isoenzyme bands have been reported in serum s...
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A flat-bed apparatus for preparative gel electrophoresis with intermittent elution [l-31 permits the collection simultaneously of anodeand cathode-migrating components of a mixnue. We employed this first for the separation of isoenzymes of lactate dehydrogenase (EC 1.1.1.27) in crude tissue extracts. Enzyme activity was determined by reaction rate observed at 340 nm in the presence of 0.14 mM N...
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1. Papadopoulos, N. M., and Hufnagel, C. A., Evaluation of lactate dehydrogenase isoenzyme patterns in serum of patients undergoing cardiac surgery. J. Thorac. Cardiovasc. Surg. 76, 173-177 (1978). 2. McKenzie, D., Clarke, P. I., and Henderson, A. R., How accurate are lactate dehydrogenase isoenzyme estimations by the thin-layer agarose fluorescent technique? Clin. Chem. 22, 1995-1998 (1976). 3...
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1. Papadopoulos, N. M., and Hufnagel, C. A., Evaluation of lactate dehydrogenase isoenzyme patterns in serum of patients undergoing cardiac surgery. J. Thorac. Cardiovasc. Surg. 76, 173-177 (1978). 2. McKenzie, D., Clarke, P. I., and Henderson, A. R., How accurate are lactate dehydrogenase isoenzyme estimations by the thin-layer agarose fluorescent technique? Clin. Chem. 22, 1995-1998 (1976). 3...
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