Gas-liquid chromatographic analysis of urinary steroids.

A semi-automated computerised gas-liquid chromatographic system for urinary steroid profile analysis.

A simple gas-liquid chromatographic method for the measurement of some of the major steroids in urine is described. The system uses conventional packed columns, automatic injection and is linked to an integrator. Calculations are all carried out by computer. The system is recommended as a simple screening procedure, producing a urinary steroid 'profile', which can process large numbers of urine...

The gas-liquid chromatography of carboxylic acid esters of the urinary 11-deoxy-17-oxo steroids. Determination as n-butyrates.

1. The gas-liquid-chromatographic separations of the acetate, propionate, n-butyrate, isobutyrate and n-valerate esters of androsterone, aetiocholanolone and dehydroepiandrosterone were studied on a 1% neopentyl glycol sebacate column. The n-butyrate, isobutyrate and n-valerate esters were well resolved. 2. The three steroids derived from hydrolysed urinary 17-oxo steroid conjugate extracts wer...

Alternative Sample Preparation Techniques in Gas Chromatographic - Mass Spectrometric Analysis of Urinary Androgenic Steroids

Evaluation of hydrolytic products of 17-ketosteroids by means of gas-liquid chromatography.

In the course of evaluating urinary 17-ketosteroid (17-KS) gas-liquid chromatographic patterns, a number of peaks with retention times shorter than that of 17-KS were constantly observed. This backgroundmaterial was found to be degradation products of 17-KS produced by HCI hydrolysis. A study with pure 17-KS as a mixture and as individual steroids was used to identify the steroids responsible f...

Acetic acid interference with determination of 5-hydroxyindoleacetic acid obviated.

1. Pratt, J. J., Steroid immunoassay in clinical chemistry. Clin. Chem. 24, 1869 (1978). 2. Shackleton, C. H. L., and Whitney, J. 0., Use of Sep-pak cartridges for urinary steroid extraction: Evaluation of the method for use prior to gas chromatographic analysis. Clin. Chim. Acta (in press). 3. Thenot, J.-P., and Horning, E. C., MOTMS derivatives of human steroids for GC and GC-MS studies. Anal...