Screening of Medium Components for Polyribosyl Ribitol Phosphate Production by Haemophilus Influenzae Type-b Using Plackett-burman Design

Plackett-Burman experimental design was used to screen important medium components influencing the production of polyribosyl ribitol phosphate by Haemophilus influenzae type-B under submerged fermentation. Eleven media parameters such as Glutamic acid, Yeast extract, cystein, dextrose, NAD, heamin, NaCl, NH4Cl, Na2HPO4, KCl, and MgSO4.7H2O were screened in twelve experimental runs as per the design. From the standard Plackett-Burman data analysis it was conformed that, heamin, yeast extract, NAD, Na2HPO4 and dextrose had contributed to a large extent, MgSO4 and L-cystein had little impact, while, glutamic acid, NH4Cl and KCl contributes moderately for polyribosyl ribitol phosphate production by haemophilus influenzae type-B under submerged fermentation.