Micro-Polymerase Chain Reaction Chip Construction for Fluorescence Detection Test

This paper proposes the optimal structure of a Printed Circuit Boardbased micro-PCR chip constructed on a PCB substrate using commercial adhesive tapes and plastic films. The proposed micro-PCR chip was composed of four layers. In a previous experiment, the chip had been made with a green PCB, yielding an unwanted reflection that prevented accurate analysis. In this study, the solder mask of the PCB substrate was coated black for a higher signal-to-noise ratio, and the area where the reaction chamber was attached was legend-printed with white silk for better reflection of the fluorescence light. The top cover was also colored black to minimize the noise during fluorescence detection. As a result of carrying out PCR, it was found that the fluorescence brightness increased with increasing numbers of cycles.