Expression of Cocoonase in Silkworm (Bombyx mori) Cells by Using a Recombinant Baculovirus and Its Bioactivity Assay
نویسندگان
چکیده
منابع مشابه
Pinellia ternata agglutinin produced in Bombyx mori cells exhibits bioactivity.
Pinellia ternata agglutinin (PTA) is highly homologous to many other monocot mannose-binding lectins which reportedly possess antitumor activities. Its production in silkworm cells has great application potential because the baculovirus expression system can produce post-translationally modified proteins at low cost. In the current study, the pta gene was cloned and expressed in silkworm cells,...
متن کاملBiological Activity of Recombinant Bovine Interferon τ Produced by a Silkworm-Baculovirus Gene Expression System
Bovine interferon (bIFN) τ plays a crucial role in maternal-fetal recognition and was expressed using a Bombyx mori (Bm) nuclear polyhedrosis virus (silkworm baculovirus) gene expression system. The biological effects of Bm-recombinant bIFNτ (rbIFNτ) on prostaglandin (PG) F2α synthesis were investigated in cultured bovine endometrial epithelial cells with oxytocin (OT, 100 nM) and on the in vit...
متن کاملExpression and localization of the spore wall protein SWP26 of Nosema bombycis in the silkworm BmN cell line
The microsporidian spore wall proteins, as the main components of the spore wall, play a key role in spore adherence to host cells and in recognition of the parasite by the host during the invasion process. In this study, we used the Bac-to-Bac baculovirus expression system to express the spore wall protein SWP26, fused to enhanced green fluorescent protein (EGFP), in the silkworm BmN cell line...
متن کاملThe silk proteins, sericin and fibroin in silkworm, Bombyx mori Linn., - a review
The domesticated silkworm, Bombyx mori Linn., a lepidopteran molecular model and an important economic insect that are emerging as an ideal molecular genetic resource for solving a broad range of biological problems. The silkworm, B. mori produces massive amount of silk proteins during the final stage of larval development. These proteins are stored in the middle silk gland and they are dischar...
متن کاملManufacturing Pharmaceutical-Grade Interferons Using Silkworm- Baculovirus System
To develop feline and canine interferons as medicine for companion animal, silkworm-baculovirus expression system was established as a manufacturing technology. Thus produced interferons were successfully cleared for a sale in commercial market. For expression of feline interferon in the silkworm, recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) was constructed carrying feline interferon cD...
متن کامل