Epitope mapping from real time kinetic studies – Role of cross- linked disulphides and incidental interacting regions in affinity measurements: Study with human chorionic gonadotropin and monoclonal antibodies NONAVINAKERE SEETHARAM SRILATHA, P TAMIL SELVI and GUNDLUPET
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Production and Characterization of Murine Monoclonal Antibodies Recognizing Conformational and Linear Epitopes Localized on Human IgA2 Molecules
Background: There are two subclasses of human IgA (IgA1 and IgA2) that differ in antigenic properties and in chemical composition. The constant domains of α1 and α2 heavy chains have >95% sequence homology though major structural differences exist in the hinge region. Quantitation of IgA subclass levels depends on the availability of monoclonal antibodies (MAbs) specific for conserved conformat...
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Real-time kinetic parameters of the interaction of iodinated human chorionic gonodotropin (IhCG) with one of its monoclonal antibodies are sought and are determined after an analysis of the dissociation profile of IhCG–MAb (monoclonal antibody) complexes in the presence of excess unlabelled hCG at various temperatures. The equilibrium association constant (KA) of the epitope–paratope reaction (...
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In this study, for the first time, a simple immunosensor for ultrasensitive recognition of Human Chorionic Gonadotropin (hCG) in serum samples was fabricated by exploiting a simple approach. In this method, a low-cost and sensitive immunosensor was fabricated based on QDs-N-S/Au nanoparticles (NPs) modified Screen-Printed Carbon Electrode (SPCE). It seems that, QDs-N-S/Au NPs/ antibody as a bio...
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Background & Aims: Antibodies, well-known as immunoglobulins (Igs), are produced by B lymphocytes and specifically defend against pathogens. Igs are glycoproteins and have high diagnostic value in several diseases including infections (1). Igs are composed of light and heavy chains (2, 3). Each chain is comprised of about 110-120 amino acid residues which create immunoglobulin folds named domai...
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