Effects of RNA interference of the eno1 gene on the malignant biological behaviors of gastric cell lines

To observe the expression of α-Enolase( ENO1) correlated with the pathological progression of gastric cancer and to investigate the effects of ENO1 down-regulation on the proliferative and migratory abilities of gastric cell lines in vitro. The expression of ENO1 was detected in 22 cases of gastric cancer, 20 cases of atypical hyperplasia and 18 cases of normal gastric tissues by immunohistochemistry. The expression level of ENO1 mRNA and protein was determined in AGS cell lines by quantitative real-time reverse transcription PCR (qRT-PCR) and Western blot, respectively. The effects of down-regulation of ENO1 on proliferative and migratory abilities of AGS cells were detected by the experiments of CCK-8, colony formation and wound healing assays. The expression level of ENO1 protein in gastric cancer tissues was higher than that of normal gastric tissues and atypical hyperplasia in the immunohistoehemical study (HC =22.70, P<0.05). It was shown from the cell proliferation curves that the proliferative ability of AGS-ENO1-siRNA transfected group was slower than that of the AGS-NC-siRNA over 72 h (t=2.50, P=0.03), meanwhile, the number of the cell-colonies of the AGS-ENO1-siRNA group were less than that of the control group after 10 days (t=22.05, P =0.01). For the abilitiy of migration, it was significantly decreased in the down-regulation ENO1 cells than in the negative cells after 48h (t=8.54, P=0.01). The results indicate that ENO1 protein may be an important potential tumor-marker associated with the development of gastric cancer.