Nucleotide sequence of rice dwarf virus genome segment 4.

نویسندگان

  • I Uyeda
  • H Kudo
  • N Yamada
  • T Matsumura
  • E Shikata
چکیده

The complete nucleotide sequence of rice dwarf virus (RDV) genome segment 4 was determined. Genome segment 4 was 2468 nucleotides long and had a long open reading frame initiating at nucleotides 64 to 66 and terminating at 2245 to 2247. The deduced polypeptide contained 727 amino acid residues with an Mr of 79.8K. Amino acid sequences similar to a 'zinc-finger' and purine NTP-binding motifs were present in the deduced polypeptide. Considerable amino acid sequence homology was detected between genome segment 4 of RDV and wound tumor virus (WTV). One of the sequences similar to the 'zinc-finger' motif was present in a conserved region of the polypeptide of both viruses. However, the sequence similar to the purine NTP-binding motif was not present in the polypeptide encoded by genome segment 4 of WTV.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Segment 5 of the rice dwarf virus genome encodes a protein highly conserved within the phytoreoviruses.

The complete nucleotide sequence of segment 5 (S5) of the rice dwarf virus (RDV) genome was determined. RDV S5 is 2571 bp in length and has a single long open reading frame which encodes a polypeptide of 801 amino acids (Mr 90495). When compared to the wound tumor virus genome S5, there was 56.9% and 52.8% similarity in the nucleotide and amino acid sequences, respectively. This high similarity...

متن کامل

Nucleotide sequence of rice dwarf virus genome segment 9.

The complete nucleotide sequence of the phytoreovirus rice dwarf virus (RDV) genome segment 9 is presented. It consisted of 1305 nucleotides and had an open reading frame that codes for a putative polypeptide of 351 amino acids. Mr of the protein was calculated to be 38,598. The terminal nucleotides 5' GGUAAA--GAU 3' were the same as those of RDV genome segment 10. The fourth nucleotide from th...

متن کامل

Sequence Analysis of Segment 8 of Five Chinese Isolates of Rice Gall Dwarf Virus and Expression of a Main Outer Capsid Protein in Escherichia coli

The rice gall dwarf disease, caused by the Rice gall dwarf virus (RGDV) is a serious disease occurring in rice in many regions of Guangdong province. As a basis to control the disease we have studied the genomic diversity of a variety of isolates from different locations. Genome segment 8(S8), encoding a main outer capsid protein (Pns8) of RGDV five isolates (BL, CH, DQ, GZ, XY) from Guangdong ...

متن کامل

The loss of outer capsid protein P2 results in nontransmissibility by the insect vector of rice dwarf phytoreovirus.

A transmission-defective (TD) isolate of rice dwarf phytoreovirus lacked the ability to infect cells when derived from the virus-free insect vector Nephotettix cincticeps. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified virus showed that among six structural proteins, the P2 outer capsid protein (encoded by genome segment S2) was absent from the TD isolate,...

متن کامل

Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses.

The complete nucleotide sequences of rice black-streaked dwarf virus (RBSDV) genome segments 8 (S8) and 7 (S7) were determined, and were found to have high sequence identities to the corresponding maize rough dwarf virus (MRDV) genome segments. RBSDV S8 and S7 consisted of 1927 and 2193 nucleotides, respectively. RBSDV S8 had a single long open reading frame (ORF) encoding 591 amino acids. RBSD...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Nucleic acids research

دوره 18 21  شماره 

صفحات  -

تاریخ انتشار 1990