Screening of nerve agent degradation products by MALDI-TOFMS.
نویسندگان
چکیده
A novel method for the rapid screening of degradation products derived from nerve agents by matrix-assisted laser desorption ionization time-of-flight mass spectrometry is described. Five standard products were selected as model compounds, including isopropyl methylphosphonic acid (IMPA), pinacolyl methylphosphonic acid (PMPA), ethyl methylphosphonic acid (EMPA), isobutyl methylphosphonic acid (i-BuMPA), and cyclohexyl methylphosphonic acid (CHMPA), which are degradation products of Sarin (GB), Soman (GD), VX, Russian VX (RVX), and GF, respectively. For comparison, CHCA (alpha-cyano-4-hydroxycinnamic acid) and DCCA (7-(diethylamino)coumarin-3-carboxylic acid) were used as the MALDI-matrix when the third harmonic generation (355 nm) of a Nd:YAG laser and a hydrogen Raman laser (multifrequency laser) were used, respectively. The method permitted the five nerve agent degradation products to be screened rapidly and successfully, suggesting that it has the potential for use as a routine monitoring tool.
منابع مشابه
Development of spray deposition/MALDI-TOFMS and its application to the rapid screening of hydrolysis products derived from nitrogen mustards.
A novel method for preparing samples for use in MALDI-TOFMS (matrix-assisted laser desorption ionization time-of-flight mass spectrometry) is described. Seven hydrolysis products derived from nitrogen mustards and CHCA (alpha-cyano-4-hydroxycinnamic acid) were selected as model compounds and the matrix, respectively. A capillary atomizer was used for evaporative and spray deposition of the samp...
متن کاملExopeptidase degradation for the analysis of phosphorylation site in a mono-phosphorylated peptide with matrix-assisted laser desorption/ionization mass spectrometry.
The utility of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) coupled with a peptide ladder sequencing method employing exopeptidase degradation for the analysis of phosphorylation site in a mono-phosphorylated peptide is investigated. MALDI-TOFMS analysis of time-dependent exopeptidase digestion using carboxypeptidase W and aminopeptidase M of the mo...
متن کاملCoupling thermal field-flow fractionation with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the analysis of synthetic polymers.
Thermal field-flow fractionation (ThFFF) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) have been coupled to yield a powerful combination of techniques for polymer analysis. Thermal FFF's high molecular weight (MW) selectivity and sensitivity to chemical composition are used to separate polydisperse polymers and polymer mixtures into the narrow po...
متن کاملSpecific recognition of DNA bulge structure by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
This study reports a novel approach utilizing an octahedral CoII(HAPP)(TFA)2 reagent in the presence of H2O2 with analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) to serve as an efficient probe for bulged DNA structures. Elucidation of DNA bulge-specific recognition pathways and cleavage mechanisms is demonstrated by characterization of bulg...
متن کاملA comparison of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and surface plasmon resonance for genotyping of high-risk human papillomaviruses.
BACKGROUND Human papillomavirus (HPV) testing coupled with appropriate clinical management is associated with a significant decline in the rate of advanced cervical cancer and associated death. METHODS In this present study, we evaluated the performance of 2 new HPV genotyping methods, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and surface plasm...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Analytical chemistry
دوره 78 13 شماره
صفحات -
تاریخ انتشار 2006