In vitro method for isolation of amastigote forms of Leishmania amazonensis from J774G8 macrophage induced by temperature shifting.
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چکیده
منابع مشابه
Role of Leishmania (Leishmania) amazonensis amastigote glycosphingolipids in macrophage infectivity.
The role of glycosphingolipids (GSLs) present in amastigote forms of Leishmania (Leishmania) amazonensis during infection of macrophages was analyzed, with particular emphasis on GSLs presenting the terminal Galpss1-3Galpa disaccharide. Macrophage invasion by L. (L.) amazonensis amastigotes was reduced by 37% when the disaccharide Galpss1-3Galp (1 mM) was added to the culture medium. The putati...
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BACKGROUND & OBJECTIVES The severe toxicity, exorbitant cost and emerging resistance of Leishmania species against most of the currently used drugs underscores the urgent need for the alternative drugs. The present study evaluates in vitro anti-leishmanial activity of Plumeria bicolor and its isolated compounds. METHODS The in vitro anti-parasitic activity of chloroform extract of Plumeria bi...
متن کاملDistinct Macrophage Fates after in vitro Infection with Different Species of Leishmania: Induction of Apoptosis by Leishmania (Leishmania) amazonensis, but Not by Leishmania (Viannia) guyanensis
Leishmania is an intracellular parasite in vertebrate hosts, including man. During infection, amastigotes replicate inside macrophages and are transmitted to healthy cells, leading to amplification of the infection. Although transfer of amastigotes from infected to healthy cells is a crucial step that may shape the outcome of the infection, it is not fully understood. Here we compare L. amazone...
متن کاملAntileishmanial activity of parthenolide, a sesquiterpene lactone isolated from Tanacetum parthenium.
The in vitro activity of parthenolide against Leishmania amazonensis was investigated. Parthenolide is a sesquiterpene lactone purified from the hydroalcoholic extract of aerial parts of Tanacetum parthenium. This isolated compound was identified through spectral analyses by UV, infrared, (1)H and (13)C nuclear magnetic resonance imaging, DEPT (distortionless enhancement by polarization transfe...
متن کاملPresence of Immunoglobulins on the Surface of Lesion-Derived Amastigotes of Leishmania major As a Tool for Isolation of Amastigote Stage from BALB/c Mice
The onset of infections by Leishmania parasites mainly caused by amastigote growth inside the macrophages. One of the important properties of lesion-derived amastigote is thought to be the attachment of various host proteins including immunoglobulins on the surface of amastigote. In this study, the presence of immunoglobulins on the surface of lesion-derived amastigotes was detected by Western ...
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ورودعنوان ژورنال:
- Memorias do Instituto Oswaldo Cruz
دوره 93 1 شماره
صفحات -
تاریخ انتشار 1998