Cytomegalovirus Towne/Toledo Chimeric Vaccines
نویسندگان
چکیده
Thomas C. Heineman, Mark Schleiss, David I. Bernstein, Richard R. Spaete, Lihan Yan, Greg Duke, Mark Prichard, Zhaoti Wang, Qing Yan, Margaret A. Sharp, Nicola Klein, Ann M. Arvin, and George Kemble Division of Infectious Diseases and Immunology, Saint Louis University School of Medicine, St. Louis, Missouri; Division of Pediatric Infectious Diseases, Children’s Hospital Medical Center, University of Cincinnati, Cincinnati, Ohio; MedImmune Vaccines, Mountain View, and Department of Pediatrics, Stanford University School of Medicine, Stanford, California; EMMES, Rockville, Maryland
منابع مشابه
A Phase 1 Study of 4 Live, Recombinant Human Cytomegalovirus Towne/Toledo Chimera Vaccines in Cytomegalovirus-Seronegative Men.
BACKGROUND Human cytomegalovirus (HCMV) infection causes disease in newborns and transplant recipients. A HCMV vaccine (Towne) protects transplant recipients. METHODS The genomes of Towne and the nonattenuated Toledo strain were recombined, yielding 4 Towne/Toledo chimera vaccines. Each of 36 HCMV-seronegative men received 1 subcutaneous dose of 10, 100, or 1000 plaque-forming units (PFU) i...
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Consistent with earlier analyses of human cytomegalovirus UL36 mRNA, we find that the UL36 protein is present throughout infection. In fact, it is delivered to the infected cell as a constituent of the virion. Curiously, much less UL36 protein accumulated in cells infected with the AD169 strain of human cytomegalovirus than in cells infected with the Towne or Toledo strain, and localization of ...
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A strain of human cytomegalovirus called Towne was isolated in WI-38 human fibrolast cell cultures from the urine of an infected infant. It was then passaged 125 times in WI-38, including three clonings, and a pool was prepared in the same cell substrate for use as a potential live attenuated vaccine. The Towne virus has a broad antigenicity and cross-reacts with the AD-169 strain. Several mark...
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In 1975, Stanley Plotkin and coworkers developed one of the first live attenuated cytomegalovirus (CMV) vaccines, called Towne 125, which was isolated from a congenitally infected infant and attenuated by cell culture passage and plaque purification [1]. Hundreds of subjects, including renal transplant recipients and healthy men and women, received the early investigational vaccine [2, 3]. The ...
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Bacterial artificial chromosome (BAC) clones have proven invaluable for genetic manipulation of herpesvirus genomes. BAC cloning can also be useful for capturing representative genomes that comprise a viral stock or mixture. The Towne live attenuated cytomegalovirus vaccine was developed in the 1970s by serial passage in cultured fibroblasts. Although its safety, immunogenicity, and efficacy ha...
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