Development of passive immunotherapy for control of infectious hematopoietic necrosis
نویسندگان
چکیده
When rainbow trout Oncorhynchus mykiss (mean weight, 4.3 g ) were injected with 100 p1 of anti-infectious hematopoietic necrosis virus (IHNV) rainbow trout immune serum (IHNV neutralizing titer 640) immediately following waterborne exposure to 10' plaque-forming units ml-' of IHNV, relative protection was 91 'X compared to fish injected with normal serum (titer < 20). Fish injected with immune serum at 24 and 48 h post-exposure to virus exhibited relative protection of 88 and 75%, respectively. Less protection was observed in fish injected 3 or 4 d post-exposure. Endogenously produced virus neutralizing titers in surviving fish injected at 0 and 24 h post-exposure were not detected but fish injected at 48 h produced a significant hulnoral response. Juvenile rainbow trout (mean weight 1 g) receiving injections of 50 p1 of serum having a low plaque neutralization titer of 20 to 40 resulted in relative protection of 67 to 82 % compared to fish injected with saline. When the same volume of a higher-titered serum was injected, greater protection was generally observed. These results suggested that low neutralization titers could be sufficient for significant protection against IHN disease. In 1 field trial, mortality due to IHN was lower in the group that received the passive irnmunotherapy compared to a group that was left untreated.
منابع مشابه
Detection and identification of white spot syndrome virus (WSSV) and infectious hypodermal and hematopoietic necrosis virus (IHHNV) of Litopenaus vannamei from Bushehr and Sistan and Baloochestan provinces (Iran), during 2009-2010
The first time the white spot disease (WSD) and IHHNV were reported in Iran was in 2004 and 2008, respectively in Bushehr Province. In Sistan and Balochestan province only the WSD was reported in 2008.The aim of this study was detecting these two viral diseases in these provinces, during December 2009 to April 2010. A total of 364 samples were collected according to suspected gross signs from...
متن کاملDetection and identification of white spot syndrome virus (WSSV) and infectious hypodermal and hematopoietic necrosis virus (IHHNV) of Litopenaus vannamei from Bushehr and Sistan and Baloochestan provinces (Iran), during 2009-2010
The first time the white spot disease (WSD) and IHHNV were reported in Iran was in 2004 and 2008, respectively in Bushehr Province. In Sistan and Balochestan province only the WSD was reported in 2008.The aim of this study was detecting these two viral diseases in these provinces, during December 2009 to April 2010. A total of 364 samples were collected according to suspected gross signs from...
متن کاملDevelopment of real-time PCR assays for detection of white spot syndrome virus, yellow head virus, Taura syndrome virus, and infectious hypodermal and hematopoietic necrosis virus in penaeid shrimp
متن کامل
Dendritic Cell Maturation with CpG for Tumor Immunotherapy
Background: Bacterial DNA has immunostimulatory effects on different types of immune cells such as dendritic cells (DCs). Application of DCs as a cellular adjuvant represents a promising approach in the immunotherapy of infectious disease and cancers. Objectives: To investigate the effect of tumor antigen pulsed DCs in the presence of CpG-1826 in treatment of a murine model of cancer. Methods: ...
متن کاملDeveloping strategies in the immunotherapy of leukemias.
BACKGROUND In the current treatment paradigms for leukemias, hematopoietic stem cell transplant (HSCT) is considered the best option with a curative potential although more often than not it simply delays disease progression. Advances are needed, both in current therapies and in the development of new strategies. Partly from studying the nuances of the curative potential of stem cell transplant...
متن کامل