Analytical validation of the Liaison hGH assay (DiaSorin)
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چکیده
Introduction Determination of the circulating growth hormone (GH) concentrations is an important tool in the diagnosis and monitoring of different pathologies, such as acromegaly and GH deficiency. However, GH determination is challenging. Indeed, the discrepancies observed between the different commercially available immunoassays represent a critical problem and have led to an important confusion in the interpretation of the GH results. This can be explained, on one hand, by the heterogeneity of the different circulating GH fragments and on the fact that these fragments are not necessarily recognized to the same extent by these kits. On the other hand, different International Standards have historically been used to calibrate GH kits. Finally, the analytical performances of these kits have generally been poorly described. Routinely, we use the Liaison hGH (DiaSorin, Saluggia, Italy) to perform GH determination. The aim of our study was to perform the analytical validation of this kit.
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