Deoxycytidine kinase from calf thymus.

Deoxycytidine kinase. II. Purification and general properties of the calf thymus enzyme.

Deoxycytidine kinase has been purified 500-fold from calf thymus. It is concluded that the enzyme will catalyze the phosphorylation of deoxycytidine, 1-P-~arabinofuranosylcytosine, deoxyguanosine, and deoxyadenosine since the protein catalyzing these activities showed coincident purification. Also, this protein could not be further fractionated by isoelectric focusing, gel filtration, or sucros...

Metabolism of deoxyribonucleotides. I. Purification and properties of deoxycytidine monophosphokinase of calf thymus.

A procedure for the partial fractionation and purification of dAMP, dGMP, dCMP, and dTMP kinases from calf thymus is described. The results obtained indicate that there are at least four distinct nucleoside monophosphokinases, specific for adenine, guanine, cytosine and uracil, and thymine. The kinase specific toward dCMP was further purified and it was shown that the stability of this enzyme w...

Pineal protein kinase: effect of enzymic phosphorylation on actinomycin D binding by, and template activity of, chromatin.

The ability of protein kinase from bovinepineal gland to phosphorylate calf-thymus chromatin and thereby to alter the association between chromatin DNA and histones was investigated. Phosphorylation of calf-thymus chromatin by pineal protein kinase results in an apparent decreased binding between the histones and DNA in chromatin, as indicated by (i) an increase in actinomycin D-binding sites a...

On the cooperative and noncooperative binding of ethidium to DNA.

The equilibrium binding of ethidium bromide to native DNAs and to poly(dG-dC) X poly(dG-dC) has been studied by both phase partition and direct spectrophotometric techniques. The binding isotherms obtained from both experimental techniques show that ethidium binds in a cooperative manner to E. coli DNA. On the other hand, no evidence of cooperative binding was observed in the binding isotherms ...

Purification and properties of calf thymus casein kinases I and II.