Rapid Rp-hplc Method with Pda Detection for Tylosin Determination in Liquid Samples

As one of the widely used veterinary macrolide antibiotics, the environmental risks of tylosin (TYL) received more and more attention. The purpose of the present study was to develop a modified, rapid and precise RP-HPLC method with PDA detection for the determination of the macrolide antibiotic tylosin in liquid samples. The proposed two HPLC methods offered short retention time (< 6.8 min), high precision (RSD ≤ 1.40 %) and linearity (R 2 > 0.9993). HPLC chromatograms exhibited well-resolved peaks with no interference peaks. Despite of the higher linearity (R 2 0.9998) of the applied HPLC Method I, the lower LOQ and LOD values of HPLC Method II indicated its better suitability for detection and quantification of low tylosin concentrations in aqueous medium (>2.78 μg/mL).