Pii: S0006-291x(05)80287-6
نویسنده
چکیده
The stoichiometric relationship between thiol oxidized and NO reduced was studied for the reaction catalyzed by nitric oxide reductase from Paracoccus denitrificans. The reaction systems consisted of dithiothreitol, ascorbate, phenazine methosulfate, enzyme and NO, or that system minus ascorbate. The mole ratio of thiol groups oxidized to NO reduced was observed to be 2.3 to 1.5 over a range of NO from 0.09 to 0.35 /~mol. A ratio of 1.0 was expected for the simple reduction of NO by 1-electron to N20. The oxidation of additional thiol is attributed to the trapping of nitrosyl hydride (nitroxyl, NO-/NOH) by thiol. ,1992 Ao~d~mio P . . . . . ~no.
منابع مشابه
Pii: 0006-291x(91)91020-d
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