Blood Coagulation Initiation
نویسنده
چکیده
Bacterial endotoxins and antigen-antibody aggregates activate complement, accelerate blood coagulation, and are implicated in intravascular blood clotting (1-7). Tha t their effect on blood coagulation proceeds through the activation of the complement system has been suspected bu t has never been shown. We have found that these and other complement-activating materials initiate blood coagulation through a complement-mediated pathway. The demonstrat ion of a coagulation abnormali ty in blood of a rabbi t deficient in the sixth component of complement (C6) and its correction with purified C6 has provided direct evidence for the involvement of complement in normal hemostasis (8). The experiments reported here indicate that activation of complement can initiate blood coagulation and suggest an important role for complement in pathologic blood coagulation.
منابع مشابه
Threshold response of initiation of blood coagulation by tissue factor in patterned microfluidic capillaries is controlled by shear rate.
OBJECTIVE Blood flow is considered one of the important parameters that contribute to venous thrombosis. We quantitatively test the relationship between initiation of coagulation and shear rate and suggest a biophysical mechanism to understand this relationship. METHODS AND RESULTS Flowing human blood and plasma were exposed to cylindrical surfaces patterned with patches of tissue factor (TF)...
متن کاملStochastic modeling of blood coagulation initiation.
A kinetic Monte Carlo simulation was developed using the deterministic reaction network developed by the Mann laboratory for tissue-factor (TF)-initiated blood coagulation. The model predicted thrombin dynamics in recalcified whole blood (3-fold diluted) pretreated with convulxin (platelet GPVI activator) and picomolar levels of TF (0-14 pM). The model did not accurately predict coagulation tim...
متن کاملBlood Coagulation Initiation by a Complement-mediated Pathway
A variety of complement-activating substances, including inulin, immunoglobulin aggregates, bacterial endotoxins, and staphylococcal protein A, were found to initiate blood coagulation through a complement-mediated pathway. These substances markedly accelerated blood coagulation in normal rabbit blood. That this clot-promoting activity requires an intact complement system was demonstrated by an...
متن کاملUsing microfluidics to understand the effect of spatial distribution of tissue factor on blood coagulation.
Initiation of blood coagulation by tissue factor (TF) is a robust, highly regulated process. Both the spatial distribution of TF and the geometry of the vasculature may play important roles in regulating coagulation. As this review describes, microfluidic systems provide a unique opportunity for investigating the spatiotemporal dynamics of blood coagulation in vitro. Microfluidic systems with s...
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Infection and inflammation are invariably associated with activation of the blood coagulation mechanism, secondary to the inflammation-induced expression of the coagulation initiator tissue factor (TF) on innate immune cells. By investigating the role of cell-surface receptors for coagulation factors in mouse endotoxemia, we found that the protein C receptor (ProcR; EPCR) was required for the n...
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