Rescue of early heart-shaped embryos and plant regeneration of Phaseolus polyanthus Greenm. and Phaseolus vulgaris L
نویسندگان
چکیده
In order to improve plant recovery from early heart-shaped Phaseolus embryos, several refinements were made to the embryo rescue technique developed previously in our laboratory. Using P. polyanthus Greenm. and P. vulgaris L. genotypes, a 20% increase in the germination rate was obtained by using Phillips salts and 25 μg.l-1 ABA. This germination medium improved the development of the embryos and increased the number of normal growing plantlets. After germination, a 2-week dehydration treatment significantly stimulated the development of heart-shaped embryos into plantlets. A high variability in terms of growth and development of the embryos was observed among the P. polyanthus genotypes. A 50% germination rate was obtained for the NI 429 genotype, compared to an average of 20% for the other genotypes.
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