Modeling of thin-layer chromatographic separation of androstane isomers

Modeling of thin-layer chromatographic separation of androstane isomers to find the optimum mobile phase is described in this paper. The isomers of androstane are present in a variety of samples, so achieving their optimum thin-layer chromatographic separation is very important. A mathematical model was developed and tested. The model takes into account the interaction between solvents and uses a complex function for modeling, so it provides reliable results. The proposed mathematical model gives results similar to those obtained by use of other optimization models, for example the ‘Simplex’ and ‘Prisma’ methods.