Influence of RAS Polymorphisms on the Development and Perinatal Outcome of Preeclampsia. Genetic RAS Evaluation

Objectives. 1. to determine the genotype distribution of Met235Thr, Thr174Met, insertion (I)/deletion (D) in the ACE gene, 18-83G/A in the REN gene, 1166A/ C in the AGTR1 gene and 3123A/G in the AGTR2 gene in women with and without PE (preeclampsia); 2. to examine the possible influence of RAS gene polymorphisms in the development and perinatal outcome of PE. Methods. Forty-five women with PE were included following inclusion and exclusion criteria. The control group comprised 51 normal pregnant women. PCRRFLP methods were used. Results. The frequency of the TT (M235T), MM (T174M) and D/D (ACE) genotypes was significantly higher (p=0.04; p=0.02; p=0.02) in female patients with PE than in female control subjects. When combined with the TT235 genotype, carriers of the MM174, D/D, AC + CC (AGTR1) or AC+ AA (AGTR2) genotypes had a 6.12; 12.5; 9.2; 5.29fold increased risk for PE. Conclusions. An increased risk of PE in women carrying the TT235, MM174 and D/D genotypes or combined TT235 and either MM174 or D/D genotypes was observed. There was a significant association of pregnancy outcome with the mutated Met235Thr (AGT), Thr174Met (AGT) and I/D (ACE) genotypes.