Investigation into the effect of varying l-leucine concentration on the product characteristics of spray-dried liposome powders.
نویسندگان
چکیده
OBJECTIVES Spray-dried formulations offer an attractive delivery system for administration of drug encapsulated into liposomes to the lung, but can suffer from low encapsulation efficiency and poor aerodynamic properties. In this paper the effect of the concentration of the anti-adherent l-leucine was investigated in tandem with the protectants sucrose and trehalose. METHODS Two manufacturing methods were compared in terms of their ability to offer small liposomal size, low polydispersity and high encapsulation of the drug indometacin. KEY FINDINGS Unexpectedly sucrose offered the best protection to the liposomes during the spray drying process, although formulations containing trehalose formed products with the best powder characteristics for pulmonary delivery; high glass transition values, fine powder fraction and yield. It was also found that l-leucine contributed positively to the characteristics of the powders, but that it should be used with care as above the optimum concentration of 0.5% (w/w) the size and polydispersity index increased significantly for both disaccharide formulations. CONCLUSIONS The method of liposome preparation had no effect on the stability or encapsulation efficiency of spray-dried powders containing optimal protectant and anti-adherent. Using l-leucine at concentrations higher than the optimum level caused instability in the reconstituted liposomes.
منابع مشابه
Investigating the effects of varying wall materials and oil loading levels on stability and nutritional values of spray dried fish oil
High oxidative capacity of polyunsaturated fatty acid rich oils is the main problem with their dietary application. The main objectives of this study were to determine the effects of different encapsulants and oil loading levels on nutritive value, fatty acid profile, and oxidative stability of microencapsulated fish oil powders. Four types of wall materials [glucose syrup and maltodextrin base...
متن کاملThe effect of polymer coatings on physicochemical properties of spray-dried liposomes for nasal delivery of BSA.
This work describes the development of spray dried polymer coated liposomes composed of soy phosphatidylcholine (SPC) and phospholipid dimyristoyl phosphatidylglycerol (DMPG) coated with alginate, chitosan or trimethyl chitosan (TMC), that are able to penetrate through the nasal mucosa and offer enhanced penetration over uncoated liposomes when delivered as a dry powder. All the liposome formul...
متن کاملFormulation optimization and characterization of spray dried microparticles for inhalation delivery of doxycycline hyclate.
The local delivery of antibiotics in the treatment of infectious respiratory diseases is an attractive alternative to deliver high concentration of antimicrobials directly to the lungs and minimize systemic side effects. In this study, inhalable microparticles containing doxycycline hyclate, sodium carboxymethylcellulose, leucine and lactose were prepared by spray drying of aqueous ethanol form...
متن کاملA Design of Experiment approach to predict product and process parameters for a spray dried influenza vaccine.
Spray dried vaccine formulations might be an alternative to traditional lyophilized vaccines. Compared to lyophilization, spray drying is a fast and cheap process extensively used for drying biologicals. The current study provides an approach that utilizes Design of Experiments for spray drying process to stabilize whole inactivated influenza virus (WIV) vaccine. The approach included systemati...
متن کاملInvestigation of oxidation behavior and Thermal spray of mechanically milled CoNiCrAlY–YSZ powders
In this study, the effects of mechanical milling and YSZ reinforcing on oxidation behavior of CoNiCrAlY coatings were investigated. Various amounts of YSZ particles (0%, 5%, 10% and 15 wt.%) were mixed with commercial CoNiCrAlY powder and milled for 24 hrs. Then, the mechanically milled and commercial powders were deposited on Inconel 617 substrate using High Velocity Oxygen-Fuel (HVOF) process...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of pharmacy and pharmacology
دوره 64 10 شماره
صفحات -
تاریخ انتشار 2012