Optimum culture conditions required for the locally isolated Dunaliella salina

Bloom of Dunaliella salina was observed naturally in stagnant puddles of water off Kuwait's Bubiyan Island on 4 July 2004. In order to closely monitor this bloom, alga were collected and maintained as pure culture of the locally isolated D. salina. Set of experiments was conducted to study and evaluate the optimum culture conditions required for two samples of D. salina (one locally isolated and the other sample brought from Perth, Australia) to achieve the highest growth performance. J. Algal Biomass Utln. 2010, 1 (2): 12-19 Optimum culture conditions for Dunaliella salina 13 The results of the experiments showed that both samples of D. salina preferred high salinities (45 psu), low temperatures (20oC), phosphate concentrations of 30 g m -3 d -3 , high light intensities (18x10 3 lux), and high pH levels of up to 9.18 (without using CO2 gas), for their optimum growth.