Flow Injection Spectrophotometric determination of Doxorubicin Hydrochloride in Urine samples

A simple and fast FI colorimetric procedure for determination of doxorubicin hydrochloride (DXH) was proposed. It is based on the redox reaction between iron (III) and DXH, resulting in an intensely red colored complex in the presence of 1, 10-orthophenantroline which can be followed by spectrophotometric measurement at λmax= 510 nm. This is an automatic way to determine doxorubicin hydrochloride concentration which is applicable to the clinical laboratories for surveying curing processes. Optimum conditions for determining doxorubicin hydrochloride were investigated by univariate method. Under the optimum conditions a linear calibration graph was obtained over the range 0.1-70 μg ml. The detection limit (3σ) and the quantification limit (10σ) were 0.03 and 0.10 μg ml of doxorubicin hydrochloride respectively. The relative standard deviation of the proposed Method for fifteen replicate injection of 40 μg ml doxorubicin hydrochloride was 0.42%. The sample throughput was 80 h. The proposed method has been successfully applied to the determination of doxorubicin hydrochloride in urine samples.