An integrated all-optical microfluidic particle sorter

A fully integrated microfluidic sorter is introduced. It is able to count, characterize and sort micrometer sized particles and cells. All functions of the device are performed by light. The objects to be sorted are counted optically, they are characterized by measuring their fluorescence. Even the sorting itself, directing the particles into different channels is performed by the pressure of light. The device is built by photopolymerization, from a light cured optically clear resin upon a glass plate support. The whole structure is created in a single photolithography step. The microfluidic channels and optical waveguides that carry the illuminating, detecting and sorting light form a single integrated structure. The supporting units, like sample reservoirs, pumps, light sources, light detectors are easily connected to the device from the outside. The device is optimized for simplicity. It is a proof-of-concept instrument, it demonstrates that it is possible to build simple optically driven microfluidic systems that perform complicated functions.