Immunocytolocalization of Glutamine Synthetase in Green Leaves and Cotyledons of Lycopersicon esculentum.

Immunocytolocalization of Ferredoxin-GOGAT in the Cells of Green Leaves and Cotyledons of Lycopersicon esculentum.

Ferredoxin-glutamate synthase was localized in leaves and cotyledons of tomato (Lycopersicon esculentum, cv Hellfrucht früshstamm) seedlings by immunocytochemical methods. The present work established that the enzyme was not only a constituent of chloroplast stroma of the mesophyll cells, but also of the cells of xylem parenchyma and epidermis.

Characterization of Glutamine Synthetase of Roots, Etiolated Cotyledons and Green Leaves from Sinapis alba (L.)

Glutamine synthetase of roots, etiolated cotyledons and green leaves from mustard plants cannot all clearly be separated by DEAE-Sephacel chromatography. However, the enzyme of the roots, etiolated cotyledons and green leaves, respectively, differed in the kinetic properties deter­ mined in the crude extract. The root enzyme showed a pH-optimum of about 6.9, a Km value of 3 m M for glutamate an...

Immunochemical comparison of glutamine synthetases from some solanaceae plants.

The presence of different glutamine synthetase isoenzymes in different Solanaceae plants and their relative antigenicities against antiglutamine synthetase from tomato leaf serum were studied. All the plants tested showed one glutamine synthetase isoenzyme except for Mandragora autumnalis, which showed two, after discontinuous polyacrylamide gel electrophoresis and specific in situ assay. Antig...

Hormones and young leaves control development of cotyledonary buds in tomato seedlings.

Hormonal and plant factors regulating the development of the inhibited cotyledonary buds of Lycopersicon esculentum Mill. cv. ;Fireball' seedlings were studied. Excision of the immature plumular leaves of 5- to 20- millimeter length significantly stimulated bud development after 2 to 4 days, but excision of leaves exceeding 20-millimeter length was without effect. Apical application of 20 micro...

Biochemical and molecular analysis of glutamine synthetase gene in brassica napus L.