Determination of new phosphorylation sites within natriuretic peptide receptors using mass spectrometric methods
نویسندگان
چکیده
Background While it has long been know that phosphorylation of the kinase homology domain is a key regulatory mechanism of natriuretic peptide receptors, no biochemical proof for individual phosphorylation sites has been reported. Here, we describe biochemical verification of previously identified phosphorylation sites in both natriuretic peptide receptor A (NPR-A) and natriuretic peptide receptor B (NPR-B) as well as the identification of novel phosphorylation sites in both receptors.
منابع مشابه
Possible identification of novel natriuretic peptide receptor phosphorylation sites by alanine/glutamate mutagenesis
Background Natriuretic peptide receptors A (NPR-A) and B (NPR-B) are transmembrane guanylyl cyclases that regulate blood pressure, heart size and long bone growth. Unlike most cell surface receptors that are desensitized by direct phosphorylation, phosphorylation of natriuretic peptide receptors is essential for activation, and dephosphorylation causes their desensitization. While there are six...
متن کاملPhosphorylation site analysis of Semliki forest virus nonstructural protein 3.
Nonstructural protein 3 (Nsp3) is an essential subunit of the alphavirus RNA replication complex, although its specific function(s) has yet to be well defined. Previously, it has been shown that Semliki Forest virus Nsp3 (482 amino acids) is a phosphoprotein, and, in the present study, we have mapped its major phosphorylation sites. Mass spectrometric methods utilized included precursor ion sca...
متن کاملFirst Report on Screening of the Profiles of the Essential Oils and Volatiles from the Aerial Parts of Marrubium persicum Using Classical and Advanced Methods Prior to Gas Chromatographic Mass Spectrometric Determination
The Lamiaceae family consists of a broad spectrum of medicinal plants involving Marrubium L. genus. Regarding the diverse pharmaceutical uses of the plants belonging to this genus, they can be considered as proper alternatives for chemical drugs having harmful effects. The present work aims to identify and characterize chemical compositions of the essential oils and volatiles from the aerial pa...
متن کاملEvaluation of Serum Levels of N-terminal Pro Brain Natriuretic Peptide and Atrial Natriuretic Peptide in Neonates with Respiratory Distress
Background: Acute respiratory distress (ARD) is a critical respiratory failure due to lung injury of neonates leading to the clinical appearance of poor lung compliance. The aimed of the study was to evaluate the diagnostic values in differentiating respiratory from heart diseases with using of N-terminal pro brain natriuretic peptide (NT-pro BNP) and Atrial natriuretic peptide(ANP) in neonates...
متن کاملMass spectrometric approaches for elucidation of antigenantibody recognition structures in molecular immunology.
Mass spectrometric approaches have recently gained increasing access to molecular immunology and several methods have been developed that enable detailed chemical structure identification of antigen-antibody interactions. Selective proteolytic digestion and MS-peptide mapping (epitope excision) has been successfully employed for epitope identification of protein antigens. In addition, "affinity...
متن کامل