Multi-channel, probe colorimeter for use with the micr-ELISA test, which makes use of disposable flat-bottom microhemagglutination plates.
نویسندگان
چکیده
We describe a multiprobe colorimeter that permits the reading of enzyme-linked immunosorbent assays (ELISA) in situ, by use of microhemagglutination plates. The use of dipping probes eliminates interference from meniscus effects and air-bubble entrapment. Detailed validation tests have been done and potential sources of inaccuracy identified.
منابع مشابه
Disposable single-use electrochemical sensor: A novel hollow fiber based tool for environmental monitoring of cadmium
The objective of this study is to design a simple, fast, sensitive and single-use electrode with the simultaneous capability of preconcentration and measuring, for application in a three-electrode voltammetry system to identify and measure the heavy metal cadmium. The design process of this sensor consists of several stages. The polyurethane foam and multi walled carbon nanotubes nanoparticles ...
متن کاملتشخیص ژن اختصاصی eae باکتری انتروپاتوژنیک اشرشیاکلی با استفاده از روش نوین PCR-ELISA
Background and purpose: Enteropathogenic Escherichia coli from Enterobacteriaceae family is one of the most common causes of chronic diarrhea in children and infants. Polymerase Chain Reaction (PCR) method is commonly used for detection of enteropathogenic Escherichia coli species, but there are some disadvantages with this method due to the use of gel electrophoresis and staining with ethidium...
متن کاملThe detection of Klebsiella pneumoniae 16srRNA specific gene by PCR-ELISA technique
Objective: Klebsiella Pneumoniae is one of the most important infection bacteria of the Enterobacteriaceae family. It is also the second factor of hospital bacteremia due to the gram negative bacteria after Escherichia coli. In this research, this bacteria with the private 16SrDNA gene, was identified through PCR-ELISA technique. Materials and Methods: In this method the dNTP labeled with Digox...
متن کاملProbe colorimeter for quantitating enzyme-linked immunosorbent assays and other colorimetric assays performed with microplates.
The performance of a fiberoptic probe colorimeter (model PC800; Brinkmann Instruments, Inc., Westbury, N.Y.) for quantitating enzymatic or colorimetric assays in 96-well microtiter plates was compared with the performances of a spectrophotometer (model 240; Gilford Instrument Laboratories, Inc., Oberlin, Ohio) and a commercially available enzyme immunoassay reader (model MR590; Dynatech Laborat...
متن کاملMethod for preparation of epidermal imprints using agarose.
about 20-fold higher compared to a conventional photometric quantification (under the same ELISA conditions with o-phenylenediamine as substrate), which reduces the use of expensive antibodies. Moreover, sophisticated detection systems like luminometers or scintillation counters for the 96-well format with capability for single-photon counting are not necessary. Thus, normal, transparent flat-b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 25 4 شماره
صفحات -
تاریخ انتشار 1979