Partition of Proteins in Aqueous Two-phase Systems Based on Cashew-nut Tree Gum and Poly(ethylene glycol)

Cell membrane abnormality detected in erythrocytes from patients with multiple sclerosis by partition in two-polymer aqueous-phase systems.

Erythrocytes from multiple sclerosis patients differ significantly (p less than 0.005) from those from controls with regard to hydrophobic affinity partition in two-polymer aqueous-phase systems containing dextran, poly(ethylene glycol) and poly (ethylene glycol)-fatty acid esters. The most likely source of the abnormality is the cell membrane.

Effective Parameters on the Partition Coefficient of Guanidine Hydrochloride in the Poly (Ethylene Glycol) +Phosphate +Water System at 298.15 K

Development and Application of Aqueous Two-Phase Partition for the Recovery and Separation of Recombinant Phenylalanine Dehydrogenase

Aqueous two-phase systems (ATPS) have  emerged as a powerful extraction method for the downstream processing of bio-molecules. The aim of this work was to investigate the possibility of utilizing ATPS for the separation of recombinant Bacillus sphaericus phenylalanine dehydrogenase (PheDH). Polyethylene glycol (PEG) and ammonium sulfate systems were selected for our experi...

Analysis of partitioning of organic compounds and proteins in aqueous polyethylene glycol-sodium sulfate aqueous two-phase systems in terms of solute-solvent interactions.

Partition behavior of nine small organic compounds and six proteins was examined in poly(ethylene glycol)-8000-sodium sulfate aqueous two-phase systems containing 0.5M osmolyte (sorbitol, sucrose, trehalose, TMAO) and poly(ethylene glycol)-10000-sodium sulfate system, all in 0.01M sodium phosphate buffer, pH 6.8. The differences between the solvent properties of the coexisting phases (solvent d...

Aqueous two-phase poly (ethylene glycol) – Maltodextrin system for protein partitioning from shrimp waste: Influence of molecular weight and pH

The polysaccharide maltodextrin (Mdx) can provide a low cost alternative to substitute the fractioned dextran for the use with polyethylene glycol (PEG) in aqueous two-phase polymer polymers systems. Aqueous two-phase system (ATPS) partitioning has been used to recover and concentrate proteins from Shrimp Waste and offers many advantages along with biomass removal. In this work we have studied ...